Success case: LAE® activity vs. Listeria monocytogenes
It has been proved that LAE® is active against L. monocytogenes in several tests as well as in its application in continuous production processes.
In vitro activity
LAE® Minimum Inhibitory Concentration (MIC) in vitro against L. monocytogenes is:
|Listeria monocytogenes B4/97||8|
|Listeria monocytogenes ATCC 15313||32|
Inoculation and superficial application
In heat-treated meat products, LAE® application as a superficial treatment reduces L. monocytogenes viability.
Result table – Inoculation
Luchansky et al. proved that the superficial application of 30 mg/kg of LAE® in 1,5 kg of ham before being vacum packed reduced L. monocytogenes incidence in 6,5 log ufc/piece in inoculated samples with 7,0 log ufc/piece. And in the case of inoculated samples with 3,0 log ufc/piece of L. monocytogenes, the application of 30 mg/kg of LAE® reduced its presence below the detection limit (1,48 log ufc/piece) during 60 days at 4 ºC.
with 7,0 log ufc/piece
|Control||LAE® at 30 mg/kg||Reduction caused by LAE® at 30 mg/kg|
|1 day at 4 ºC||6,9 log ufc/piece||<1,48 log ufc/piece||>5,42 log ufc/piece|
|Ham Inoculation with 3,0 log ufc/piece||Control||LAE®at 30 mg/kg||Redution caused by LAE® at
|1 day at 4 ºC||3,4 log ufc/piece||<1,48 log ufc/piece||>1,92 log ufc/piece
|60 days at 4 ºC||4,7 log ufc/piece||<1,48 log ufc/piece||>3,22 log ufc/piece|
Result table – Superficial application
The ABC Research (USA) laboratory has carried out tests in different sliced cooked ham products and sliced smoked turkey, applying LAE® superficially at approximately 160 mg/kg resulting in a noticeable initial reduction of L. monocytogenes of 1,63 log ufc/g and >1,94 log ufc/g respectively.
|Initial counting||Control||LAE® at 200 mg/Kg||Redution caused by LAE® at 200 mg/Kg|
|Sliced cooked ham||3,67 log ufc/g||2,04 log ufc/g||1,63 log ufc/g|
|Sliced smoked turkey||3,04 log ufc/g||<1,10 log ufc/g||>1,94 log ufc/g|
Stopforth et al. indicated that the superficial application of 70 mg/kg of LAE® in 70 g ham cooked slices inoculated with 3,3 log ufc/g of L. monocytogenes, reduces to 1 log ufc/g L. monocytogenes presence.